Tuesday, 28 November 2006

molecular biology - Should I design a primer to detect virus based on NCBI genebank?


Any other database better than NCBI ?




A lot of people overlook some sub-databases inside NCBI and search the main nucleotide database only.



If You never tried it, you can blast the HIV sequence which You have found, against other databases. On the ncbi blast webpage, just choose the options other than nucleotide collection (nr/nt). I tend to find new sequences in Expressed sequence tags (EST), Genomic survey sequences (GSS) or Whole genome shotgun contigs (WGS). Some of these databases cover unfinished sequencing project and should be a source of new data.



(I am a plant person, not virus person, so maybe you will find out, that different databases are worth searching for you. Or you will find it a waste of time.)



BTW, now some time had passed since your question. How did you solve Your HIV sequence problem ?

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