The answer is no. Here is a counter-example
left( begin{matrix} 0 & -5 \\ 1 & 0 end{matrix} right) quad mbox{and} quad left( begin{matrix} -1 & -3 \\ 2 & 1 end{matrix} right).
Both of these matrices have characteristic polynomial x2+5. For pneq2, 5, this polynomial has no repeated factors so any matrices with this polynomial are similar. By the same argument, they are similar over mathbbQ. By brute force computation, they are also similar at 2 and 5.
However, they are not similar over mathbbZ. Consider mathbbZ2 as a module for mathbbZ[t] where t acts by one of the two matrices above. Both of these matrices square to −5, so these are in fact mathbbZ[sqrt−5]-modules. If the matrices were similar, the similarity would give an isomorphism of mathbbZ[sqrt−5]-modules. But these are not isomorphic: the former is free on one generator while the latter is isomorphic to the ideal langle2,1+sqrt−5rangle.
In general, the way to classify similarity of matrices over mathbbZ is the following: If the matrices do not have the same characteristic polynomial over mathbbQ, they are not similar. If they do, let f be the characteristic polynomial and let R=mathbbZ[t]/f(t). Then your matrices give R-modules, and the matrices are similar if and only if the R-modules are isomorphic. If R is the ring of integers of a number field, then R-modules are classified by the ideal class group. In general, they are related to the ideal class group, but there are various correction factors related to how R fails to be the ring of integers of its fraction field (or how it fails to be a domain at all). I don't know the details here.
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