Sunday, 4 May 2008

Attaching suspension cells to coverslip during mycoplasma contamination detection

I am planing to screen my cell cultures for mycoplasma contamination using the Hoechst 33258 DNA staining method.



This method is suitable for adherent cultures. Is this method suitable for suspension cultures?



If yes, what is the procedure to attach my suspension culture on coverslip and prevent washout of cells during staining.

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