biochemistry - Which concentration of BSA is recommended for dynamic light scattering experiments?

You can easly calculate the absorbance from Beer's Law

A = εlc

Here you can get the values for Extinction coefficient by sequence at http://web.expasy.org/cgi-bin/protparam/protparam1?P02769@25-607@

So for your example 0.001 g/L 3 g/L with this R code, you can get the absorbance values for each concentration.

> PM <- 66432.9 #g/mol
> c <- seq(0.001,3,0.05) #get values from 0.001 to 3, by 0.05 increment
> V <- 1 #liters
> M = c((c/PM)/V)
> #Lambert Beer Law
> e <- 42925
> l <- 1
> A <- c(e*l*M)
> plot(A ~ c, ylab="A (280nm)", xlab="g/l")
> data.frame("conc"=c, M=M, A=A)
    conc            M            A
1  0.001 1.505278e-08 0.0006461407
2  0.051 7.676919e-07 0.0329531753
3  0.101 1.520331e-06 0.0652602099
4  0.151 2.272970e-06 0.0975672445
5  0.201 3.025609e-06 0.1298742792
6  0.251 3.778248e-06 0.1621813138
7  0.301 4.530888e-06 0.1944883484
8  0.351 5.283527e-06 0.2267953830
9  0.401 6.036166e-06 0.2591024176
10 0.451 6.788805e-06 0.2914094522
11 0.501 7.541444e-06 0.3237164869
12 0.551 8.294083e-06 0.3560235215
13 0.601 9.046722e-06 0.3883305561
14 0.651 9.799361e-06 0.4206375907
15 0.701 1.055200e-05 0.4529446253
16 0.751 1.130464e-05 0.4852516599
17 0.801 1.205728e-05 0.5175586946
18 0.851 1.280992e-05 0.5498657292
19 0.901 1.356256e-05 0.5821727638
20 0.951 1.431520e-05 0.6144797984
21 1.001 1.506784e-05 0.6467868330
22 1.051 1.582047e-05 0.6790938676
23 1.101 1.657311e-05 0.7114009023
24 1.151 1.732575e-05 0.7437079369
25 1.201 1.807839e-05 0.7760149715
26 1.251 1.883103e-05 0.8083220061
27 1.301 1.958367e-05 0.8406290407
28 1.351 2.033631e-05 0.8729360753
29 1.401 2.108895e-05 0.9052431100
30 1.451 2.184159e-05 0.9375501446
31 1.501 2.259423e-05 0.9698571792
32 1.551 2.334687e-05 1.0021642138
33 1.601 2.409950e-05 1.0344712484
34 1.651 2.485214e-05 1.0667782830
35 1.701 2.560478e-05 1.0990853177
36 1.751 2.635742e-05 1.1313923523
37 1.801 2.711006e-05 1.1636993869
38 1.851 2.786270e-05 1.1960064215
39 1.901 2.861534e-05 1.2283134561
40 1.951 2.936798e-05 1.2606204908
41 2.001 3.012062e-05 1.2929275254
42 2.051 3.087326e-05 1.3252345600
43 2.101 3.162590e-05 1.3575415946
44 2.151 3.237854e-05 1.3898486292
45 2.201 3.313117e-05 1.4221556638
46 2.251 3.388381e-05 1.4544626985
47 2.301 3.463645e-05 1.4867697331
48 2.351 3.538909e-05 1.5190767677
49 2.401 3.614173e-05 1.5513838023
50 2.451 3.689437e-05 1.5836908369
51 2.501 3.764701e-05 1.6159978715
52 2.551 3.839965e-05 1.6483049062
53 2.601 3.915229e-05 1.6806119408
54 2.651 3.990493e-05 1.7129189754
55 2.701 4.065757e-05 1.7452260100
56 2.751 4.141020e-05 1.7775330446
57 2.801 4.216284e-05 1.8098400792
58 2.851 4.291548e-05 1.8421471139
59 2.901 4.366812e-05 1.8744541485
60 2.951 4.442076e-05 1.9067611831

And you can get this plot from 0.001 to 3 g/l for albumine

Now you can choose the desired concentration for the absorbance you wanna work with